May Grunwald Giemsa for sections 100 test

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Product for the preparation of cyto-histological samples for optical microscopy. Recommended method to differentiate cell types and to reveal parasites in tissue sections. Especially useful for lymphopoietic tissue. This stain is often used to demonstrate endothelial reticulum.

- May Grunwald solution, consisting of eosin-methylene blue, stains nuclei blue and basophil cytoplasm pinkish red.
- Giemsa solution, a complex consisting of methylene blue chloride, eosin-methylene blue and azure II eosinate, improves the intensity of nuclear staining and the capacity to show selectively cellular structures. To appreciate results always remember two factors: pH of washing water and dilution buffer have a strong influence on final colour chart; intensity of stain may vary according to differentiation time.

METHOD:

1) Deparaffinise section and bring to ethanol 70°.
2) Preparation of buffer solution: in the enclosed capsule introduce 20 ml of distilled water; add 10 drops of concentrated solution B. This solution is called “buffer solution B” in the method.
3) Put on the section 10 drops of buffer solution B: leave to act 2 minutes.
4) Drain the slide and put 10 drops of reagent A and 5 drops of buffer solution B: leave to act 5 minutes.
5) Pipette 10 ml of buffer solution B and wash carefully the slide in this solution.
6) Put in capsule 5 drops of reagent C and 10 drops of buffer solution B, shake the solution and put it on the slide: leave to act 12 minutes.
7) Differentiate in: Ethanol 95° 10 seconds Absolute ethanol 30 seconds Absolute ethanol 30 seconds.
8) Clear in xylene and mount.