Silver Methenamine - P.A.S.M. 100 test - Special Stains

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Product for the preparation of cyto-histological samples for optical microscopy. To show argyrophilic elements and mucopolysaccharides (basal membranes, mycetes, bacteria etc.) in tissue sections. Recommended method to examine basal membrane in renal biopsy.

Method:

1) Bring section to distilled water.
2) Put on the section 10 drops of reagent A: leave to act for 30 minutes.
3) Rinse in distilled water.
4) Prepare the incubation box and lay down the slide; pour 10 drops of reagent B into the vial, add 10 drops of reagent C and 10 drops of reagent D, shake and put the obtained solution on the section, close the incubation box and incubate in oven at 60° C for 30-40 minutes.
5) Take out the incubation box from the oven and check the tone of impregnation, if it is correct wait 5 minutes and rinse in distilled water. If it is weak, reincubate in the oven and check every 5 minutes.
6) Put on the section 10 drops of reagent E: leave to act 1 minute.
7) Rinse in distilled water.
8) Put on the section 10 drops of reagent F: leave to act 1 minute.
9) Rinse in distilled water.
10)Dehydrate through ascending alcohols, clear in xylene and mount.